## The **weight average sedimentation coefficient** can be computed from the g(s*) curves by the following relationship:

## Other averages (moments) of s are defined by the following relations:

## The **diffusion coefficient** can be determined from the standard deviation of a gaussian curve fit to the time derivative profile.

Although A is a function of s*, it can be treated as a constant for the purposes of estimating D since it doesn't noticeably effect the estimation of the width. (s is the the same as s*-peak and is the sedimentation coefficient of the macromolecule; remember that s* is the transformed x-axis in the moving reference frame.)